1GGN

Structures of glycogen phosphorylase-inhibitor complexes and the implications for structure-based drug design

1GGN の概要

• エントリーDOI: 10.2210/pdb1ggn/pdb
• 関連するPDBエントリー: 1A8I
• 分子名称: PROTEIN (GLYCOGEN PHOSPHORYLASE), BETA-D-GLUCOPYRANOSE SPIROHYDANTOIN, PYRIDOXAL-5'-PHOSPHATE, ... (4 entities in total)
• 機能のキーワード: transferase, glycogen phosphorylase, inhibitor complex, catalytic site, design
• 由来する生物種: Oryctolagus cuniculus (rabbit)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 97786.54

構造登録者

Watson, K.A.,Tsitsanou, K.E.,Gregoriou, M.,Zographos, S.E.,Skamnaki, V.T.,Oikonomakos, N.G.,Fleet, G.W.,Johnson, L.N. (登録日: 2000-08-29, 公開日: 2000-09-13, 最終更新日: 2023-08-09)

主引用文献

Oikonomakos, N.G.,Skamnaki, V.T.,Osz, E.,Szilagyi, L.,Somsak, L.,Docsa, T.,Toth, B.,Gergely, P.
Kinetic and crystallographic studies of glucopyranosylidene spirothiohydantoin binding to glycogen phosphorylase B.
Bioorg.Med.Chem., 10:261-268, 2002

PubMed Abstract: Glucopyranosylidene spirothiohydantoin (TH) has been identified as a potential inhibitor of both muscle and liver glycogen phosphorylase b (GPb) and a (GPa) and shown to diminish liver GPa activity in vitro. Kinetic experiments reported here show that TH inhibits muscle GPb competitively with respect to both substrates phosphate (K(i)=2.3 microM) and glycogen (K(i)=2.8 microM). The structure of the GPb-TH complex has been determined at a resolution of 2.26 A and refined to a crystallographic R value of 0.193 (R(free)=0.211). The structure of GPb-TH complex reveals that the inhibitor can be accommodated in the catalytic site of T-state GPb with very little change of the tertiary structure, and provides a basis of understanding potency and specificity of the inhibitor. The glucopyranose moiety makes the standard hydrogen bonds and van der Waals contacts as observed in the glucose complex, while the rigid thiohydantoin group is in a favourable electrostatic environment and makes additional polar contacts to the protein.

PubMed: 11741774
DOI: 10.1016/S0968-0896(01)00277-2

実験手法

X-RAY DIFFRACTION (2.36 Å)