1GG0

CRYSTAL STRUCTURE ANALYSIS OF KDOP SYNTHASE AT 3.0 A

1GG0 の概要

• エントリーDOI: 10.2210/pdb1gg0/pdb
• 関連するPDBエントリー: 1D9E 1GG1
• 分子名称: 3-DEOXY-D-MANNO-OCTULOSONATE 8-PHOSPHATE SYNTHASE, PHOSPHATE ION (2 entities in total)
• 機能のキーワード: beta-alpha-barrel, lyase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P0A715
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31060.62

構造登録者

Wagner, T.,Kretsinger, R.H.,Bauerle, R.,Tolbert, W.D. (登録日: 2000-08-04, 公開日: 2000-10-04, 最終更新日: 2023-12-27)

主引用文献

Wagner, T.,Kretsinger, R.H.,Bauerle, R.,Tolbert, W.D.
3-Deoxy-D-manno-octulosonate-8-phosphate synthase from Escherichia coli. Model of binding of phosphoenolpyruvate and D-arabinose-5-phosphate.
J.Mol.Biol., 301:233-238, 2000

PubMed Abstract: The crystal structure of 3-deoxy-d-manno-octulosonate-8-phosphate synthase (KDOPS) from Escherichia coli was determined by molecular replacement using coordinates given to us by Radaev and co-workers prior to publication. The KDOPS crystals reported by Radaev et al. were grown in the presence of 1.4 M (NH(4))(2)SO(4) and 0.4 M (K/H)(3)PO(4). They are in the cubic space group I23 (a=228.6 A) with a tetramer in the asymmetric unit; the structure has been refined with data to 2.4 A. Our crystals of E. coli KDOPS, grown in 24 % (w/v) polyethylene glycol (PEG) 1500 in the presence of the substrates, 2-phosphoenolpyruvate (PEP) and d-arabinose-5-phosphate (A5P), are also in space group I23 (a=118.2 A), with one subunit in the asymmetric unit. The medium of crystallization, 1.8 M SO(4)/PO(4) versus 24 % PEG, does not significantly affect the conformation of KDOPS. The inter-monomer contacts in both structures are the same. The beta(8)/alpha(8) loop (residues 246 to 251) situated near the entrance to the active site is not seen in the 229 A structure but can be traced in the 118 A structure. Most significantly, Radaev et al. interpreted two SO(4)/PO(4) sites in the 229 A structure as marking the phosphate positions of the substrates, PEP and A5P, after the precedent of DAHPS. In the 118 A structure the inner of these two SO(4)/PO(4) peaks is present at the same position as in the 229 A structure of KDOPS. The outer phosphate peak in the 118 A KDOPS is 3.7 A from the outer SO(4)/PO(4) peak in the 229 A structure and is within hydrogen bonding distance of Arg63 of the same subunit and Arg120 of another subunit. Based on the precedent of the d-erythrose-4-phosphate (E4P) modeled in the active site of DAHPS, we have modeled PEP and A5P in KDOPS and compared the coordination of PEP and A5P in KDOPS with that of PEP and E4P in DAHPS.

PubMed: 10926505
DOI: 10.1006/jmbi.2000.3956

実験手法

X-RAY DIFFRACTION (3 Å)