1GFA

CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS

Summary for 1GFA

• Entry DOI: 10.2210/pdb1gfa/pdb
• Related: 1GAY 1GF8 1GF9 1GFE 1GFG 1GFH 1GFJ 1GFK 1GFR 1GFT 1GFU 1GFV 1INU
• Descriptor: LYSOZYME, SODIUM ION (3 entities in total)
• Functional Keywords: surface, hydrophilic, stability, hydrolase
• Biological source: Homo sapiens (human)
• Cellular location: Secreted: P61626
• Total number of polymer chains: 1
• Total formula weight: 14759.64

Authors

Funahashi, J.,Takano, K.,Yamagata, Y.,Yutani, K. (deposition date: 2000-12-04, release date: 2000-12-20, Last modification date: 2023-12-27)

Primary citation

Funahashi, J.,Takano, K.,Yamagata, Y.,Yutani, K.
Positive contribution of hydration structure on the surface of human lysozyme to the conformational stability.
J.Biol.Chem., 277:21792-21800, 2002

PubMed Abstract: Water molecules make a hydration structure with the network of hydrogen bonds, covering on the surface of proteins. To quantitatively estimate the contribution of the hydration structure to protein stability, a series of hydrophilic mutant human lysozymes (Val to Ser, Tyr, Asp, Asn, and Arg) modified at three different positions on the surface, which are located in the alpha-helix (Val-110), the beta-sheet (Val-2), and the loop (Val-74), were constructed. Their thermodynamic parameters of denaturation and crystal structures were examined by calorimetry and by x-ray crystallography at 100 K, respectively. The introduced polar residues made hydrogen bonds with protein atoms and/or water molecules, sometimes changing the hydration structure around the mutation site. Changes in the stability of the mutant proteins can be evaluated by a unique equation that considers the conformational changes resulting from the substitutions. Using this analysis, the relationship between the changes in the stabilities and the hydration structures for mutant human lysozymes substituted on the surface could be quantitatively estimated. The analysis indicated that the hydration structure on protein surface plays an important role in determining the conformational stability of the protein.

PubMed: 11927576
DOI: 10.1074/jbc.M110728200

Experimental method

X-RAY DIFFRACTION (1.8 Å)