1GDR

MODEL FOR A DNA MEDIATED SYNAPTIC COMPLEX SUGGESTED BY CRYSTAL PACKING OF GAMMA DELTA RESOLVASE SUBUNITS

1GDR の概要

• エントリーDOI: 10.2210/pdb1gdr/pdb
• 分子名称: GAMMA DELTA-RESOLVASE (1 entity in total)
• 機能のキーワード: site-specific recombinase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15515.83

構造登録者

Rice, P.A.,Steitz, T.A. (登録日: 1993-08-31, 公開日: 1994-04-30, 最終更新日: 2024-02-07)

主引用文献

Rice, P.A.,Steitz, T.A.
Model for a DNA-mediated synaptic complex suggested by crystal packing of gamma delta resolvase subunits.
EMBO J., 13:1514-1524, 1994

PubMed Abstract: The packing arrangement of the 12 subunits of intact gamma delta resolvase in the unit cell of a hexagonal crystal form suggests a model for site-specific recombination that involves a DNA-mediated synaptic intermediate. The crystal structure has been determined by molecular replacement and partially refined at 2.8/3.5 A resolution. Although the small DNA-binding domain is disordered in these crystals, packing considerations show that only a small region of space in the crystal could accommodate a domain of its size. A family of related models for a synaptic complex between two DNA duplexes and 12 monomers that are arranged as situated in the crystal is consistent with the known topology of the complex and the distances between the three resolvase dimer-binding sites per DNA; further, these models place the two DNA recombination sites in contact with each other between two resolvase dimers, implying that strand exchange is accomplished through direct DNA-DNA interaction. A major role postulated, then, for the resolvase protein assembly is to stabilize a res DNA structure that is close to the topological transition state of the reaction.

PubMed: 8156989

実験手法

X-RAY DIFFRACTION (3.5 Å)