1GCY

HIGH RESOLUTION CRYSTAL STRUCTURE OF MALTOTETRAOSE-FORMING EXO-AMYLASE

1GCY の概要

• エントリーDOI: 10.2210/pdb1gcy/pdb
• 分子名称: GLUCAN 1,4-ALPHA-MALTOTETRAHYDROLASE, CALCIUM ION (3 entities in total)
• 機能のキーワード: beta-alpha-barrel, beta sheet, hydrolase
• 由来する生物種: Pseudomonas stutzeri
• 細胞内の位置: Secreted: P13507
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 57849.16

構造登録者

Mezaki, Y.,Katsuya, Y.,Kubota, M.,Matsuura, Y. (登録日: 2000-08-14, 公開日: 2000-08-30, 最終更新日: 2024-10-23)

主引用文献

Mezaki, Y.,Katsuya, Y.,Kubota, M.,Matsuura, Y.
Crystallization and structural analysis of intact maltotetraose-forming exo-amylase from Pseudomonas stutzeri.
Biosci.Biotechnol.Biochem., 65:222-225, 2001

PubMed Abstract: The intact maltotetraose-forming exo-amylase from Pseudomonas stutzeri (G4-1), which has a raw starch binding domain, has been crystallized. The structure was identified (PDB entry 1GCY) by the molecular replacement method using the structure of its catalytic domain (G4-2). The result showed that the raw starch binding domain is in a disordered state, the corresponding electron densities being almost invisible. Superposition of these two enzyme forms showed evidence for the possible location of the raw starch binding domain (SBD). This crystal is a novel case, in that it forms a regular lattice incorporating flexibly bound SBD in the channel of crystal packing of the catalytic domains.

PubMed: 11272837
DOI: 10.1271/bbb.65.222

実験手法

X-RAY DIFFRACTION (1.6 Å)