1G9U

CRYSTAL STRUCTURE OF YOPM-LEUCINE RICH EFFECTOR PROTEIN FROM YERSINIA PESTIS

1G9U の概要

• エントリーDOI: 10.2210/pdb1g9u/pdb
• 関連するPDBエントリー: 1d0b 1dfj
• 分子名称: OUTER PROTEIN YOPM, ACETATE ION, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: toxin
• 由来する生物種: Yersinia pestis
• 細胞内の位置: Cell outer membrane: P17778
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53804.92

構造登録者

Evdokimov, A.G.,Anderson, D.E.,Routzahn, K.M.,Waugh, D.S. (登録日: 2000-11-28, 公開日: 2001-10-10, 最終更新日: 2024-04-03)

主引用文献

Evdokimov, A.G.,Anderson, D.E.,Routzahn, K.M.,Waugh, D.S.
Unusual molecular architecture of the Yersinia pestis cytotoxin YopM: a leucine-rich repeat protein with the shortest repeating unit.
J.Mol.Biol., 312:807-821, 2001

PubMed Abstract: Many Gram-negative bacterial pathogens employ a contact-dependent (type III) secretion system to deliver effector proteins into the cytosol of animal or plant cells. Collectively, these effectors enable the bacteria to evade the immune response of the infected organism by modulating host-cell functions. YopM, a member of the leucine-rich repeat protein superfamily, is an effector produced by the bubonic plague bacterium, Yersinia pestis, that is essential for virulence. Here, we report crystal structures of YopM at 2.4 and 2.1 A resolution. Among all leucine-rich repeat family members whose atomic coordinates have been reported, the repeating unit of YopM has the least canonical secondary structure. In both crystals, four YopM monomers form a hollow cylinder with an inner diameter of 35 A. The domain that targets YopM for translocation into eukaryotic cells adopts a well-ordered, alpha-helical conformation that packs tightly against the proximal leucine-rich repeat module. A similar alpha-helical domain can be identified in virulence-associated leucine-rich repeat proteins produced by Salmonella typhimurium and Shigella flexneri, and in the conceptual translation products of several open reading frames in Y. pestis.

PubMed: 11575934
DOI: 10.1006/jmbi.2001.4973

実験手法

X-RAY DIFFRACTION (2.35 Å)