1G92

SOLUTION STRUCTURE OF PONERATOXIN

1G92 の概要

• エントリーDOI: 10.2210/pdb1g92/pdb
• NMR情報: BMRB: 4921
• 分子名称: PONERATOXIN (1 entity in total)
• 機能のキーワード: toxin; neurotoxin; sodium channel inhibitor, toxin
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2759.36

構造登録者

Szolajska, E.,Poznanski, J.,Ferber, M.L.,Michalik, J.,Gout, E.,Fender, P.,Bailly, I.,Dublet, B.,Chroboczek, J. (登録日: 2000-11-22, 公開日: 2003-11-18, 最終更新日: 2024-05-22)

主引用文献

Szolajska, E.,Poznanski, J.,Ferber, M.L.,Michalik, J.,Gout, E.,Fender, P.,Bailly, I.,Dublet, B.,Chroboczek, J.
Poneratoxin, a neurotoxin from ant venom. Structure and expression in insect cells and construction of a bio-insecticide.
Eur.J.Biochem., 271:2127-2136, 2004

PubMed Abstract: Poneratoxin is a small neuropeptide found in the venom of the ant Paraponera clavata. It is stored in the venom reservoir as an inactive 25-residue peptide. Here we describe both chemically synthesized poneratoxin and poneratoxin obtained by expression in insect cells. When expressed in insect cells, poneratoxin was observed attached to cell membranes. Both synthetic and recombinant ponerotoxins were soluble below pH 4.5. The structure of synthetic poneratoxin was characterized by circular dichroism and solved by nuclear magnetic resonance. In an environment imitating a lipid bilayer, at pH within the range of insect hemolymph, synthetic poneratoxin has a V shape, with two alpha-helices connected by a beta-turn. Insect larvae were paralyzed by injection of either of the purified toxins, with the recombinant one acting faster. The recombinant toxin-producing baculovirus reduced the average survival time of the insect host by 25 h compared with unmodified virus. Mass spectrometry analysis showed that the recombinant toxin has an N-terminal 21-residue extension, possibly improving its stability and/or stabilizing the membrane-bound state. The potential use of poneratoxin for the construction of biological insecticide is discussed.

PubMed: 15153103
DOI: 10.1111/j.1432-1033.2004.04128.x

実験手法

SOLUTION NMR