1G8Z

HIS57ALA MUTANT OF CHOLERA TOXIN B-PENATMER

1G8Z の概要

• エントリーDOI: 10.2210/pdb1g8z/pdb
• 関連するPDBエントリー: 2CHB 3CHB
• 分子名称: CHOLERA TOXIN B PROTEIN, beta-D-galactopyranose (3 entities in total)
• 機能のキーワード: toxin
• 由来する生物種: Vibrio cholerae
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 58501.61

構造登録者

Aman, A.T.,Fraser, S.,Merritt, E.A.,Rodigherio, C.,Kenny, M. (登録日: 2000-11-21, 公開日: 2001-07-25, 最終更新日: 2024-10-30)

主引用文献

Aman, A.T.,Fraser, S.,Merritt, E.A.,Rodigherio, C.,Kenny, M.,Ahn, M.,Hol, W.G.,Williams, N.A.,Lencer, W.I.,Hirst, T.R.
A mutant cholera toxin B subunit that binds GM1- ganglioside but lacks immunomodulatory or toxic activity.
Proc.Natl.Acad.Sci.USA, 98:8536-8541, 2001

PubMed Abstract: GM1-ganglioside receptor binding by the B subunit of cholera toxin (CtxB) is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. More recently, GM1 binding by isolated CtxB, or the related B subunit of Escherichia coli heat-labile enterotoxin (EtxB), has been found to modulate leukocyte function, resulting in the down-regulation of proinflammatory immune responses that cause autoimmune disorders such as rheumatoid arthritis and diabetes. Here, we demonstrate that GM1 binding, contrary to expectation, is not sufficient to initiate toxin action. We report the engineering and crystallographic structure of a mutant cholera toxin, with a His to Ala substitution in the B subunit at position 57. Whereas the mutant retained pentameric stability and high affinity binding to GM1-ganglioside, it had lost its immunomodulatory activity and, when part of the holotoxin complex, exhibited ablated toxicity. The implications of these findings on the mode of action of cholera toxin are discussed.

PubMed: 11447291
DOI: 10.1073/pnas.161273098

実験手法

X-RAY DIFFRACTION (2 Å)