1G5H

CRYSTAL STRUCTURE OF THE ACCESSORY SUBUNIT OF MURINE MITOCHONDRIAL POLYMERASE GAMMA

1G5H の概要

• エントリーDOI: 10.2210/pdb1g5h/pdb
• 関連するPDBエントリー: 1G5I
• 分子名称: MITOCHONDRIAL DNA POLYMERASE ACCESSORY SUBUNIT, SODIUM ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: intermolecular four helix bundle, dna binding protein
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Mitochondrion: Q9QZM2
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 205621.29

構造登録者

Carrodeguas, J.A.,Theis, K.,Bogenhagen, D.F.,Kisker, C. (登録日: 2000-11-01, 公開日: 2001-03-14, 最終更新日: 2024-10-09)

主引用文献

Carrodeguas, J.A.,Theis, K.,Bogenhagen, D.F.,Kisker, C.
Crystal structure and deletion analysis show that the accessory subunit of mammalian DNA polymerase gamma, Pol gamma B, functions as a homodimer.
Mol.Cell, 7:43-54, 2001

PubMed Abstract: Polymerase gamma, which replicates and repairs mitochondrial DNA, requires the Pol gamma B subunit for processivity. We determined the crystal structure of mouse Pol gamma B, a core component of the mitochondrial replication machinery. Pol gamma B shows high similarity to glycyl-tRNA synthetase and dimerizes through an unusual intermolecular four-helix bundle. A human Pol gamma B mutant lacking the four-helix bundle failed to dimerize in solution or to stimulate the catalytic subunit Pol gamma A, but retained the ability to bind with Pol gamma A to a primer-template construct, indicating that the functional holoenzyme contains two Pol gamma B molecules. Other mutants retained stimulatory activity but lost the ability to bind folded ssDNA. These results suggest that the Pol gamma B dimer contains distinct sites for Pol gamma A binding, dimerization, and DNA binding.

PubMed: 11172710
DOI: 10.1016/S1097-2765(01)00153-8

実験手法

X-RAY DIFFRACTION (1.95 Å)