1G57

CRYSTAL STRUCTURE OF 3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE

1G57 の概要

• エントリーDOI: 10.2210/pdb1g57/pdb
• 関連するPDBエントリー: 1G58
• 分子名称: 3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE, CESIUM ION (3 entities in total)
• 機能のキーワード: dihydroxybutanone phosphate synthase, riboflavine biosynthesis, skeletal rearrangement, antimicrobial target, structure-based design, isomerase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell membrane; Peripheral membrane protein (Potential): P0A7J0
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47202.49

構造登録者

Liao, D.-I.,Calabrese, J.C.,Wawrzak, Z.,Viitanen, P.V.,Jordan, D.B. (登録日: 2000-10-30, 公開日: 2001-04-30, 最終更新日: 2024-02-07)

主引用文献

Liao, D.I.,Calabrese, J.C.,Wawrzak, Z.,Viitanen, P.V.,Jordan, D.B.
Crystal structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase of riboflavin biosynthesis.
Structure, 9:11-18, 2001

PubMed Abstract: 3,4-Dihydroxy-2-butanone-4-phosphate synthase catalyzes a commitment step in the biosynthesis of riboflavin. On the enzyme, ribulose 5-phosphate is converted to 3,4-dihydroxy-2-butanone 4-phosphate and formate in steps involving enolization, ketonization, dehydration, skeleton rearrangement, and formate elimination. The enzyme is absent in humans and an attractive target for the discovery of antimicrobials for pathogens incapable of acquiring sufficient riboflavin from their hosts. The homodimer of 23 kDa subunits requires Mg(2+) for activity.

PubMed: 11342130
DOI: 10.1016/S0969-2126(00)00550-5

実験手法

X-RAY DIFFRACTION (1.4 Å)