1G3J

CRYSTAL STRUCTURE OF THE XTCF3-CBD/BETA-CATENIN ARMADILLO REPEAT COMPLEX

1G3J の概要

• エントリーDOI: 10.2210/pdb1g3j/pdb
• 分子名称: BETA-CATENIN ARMADILLO REPEAT REGION, TCF3-CBD (CATENIN BINDING DOMAIN) (3 entities in total)
• 機能のキーワード: beta-catenin, tcf-3, protein-protein complex, transcription
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: P35222 P70062
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 128993.80

構造登録者

Graham, T.A.,Weaver, C.,Mao, F.,Kimelman, D.,Xu, W. (登録日: 2000-10-24, 公開日: 2000-12-11, 最終更新日: 2024-02-07)

主引用文献

Graham, T.A.,Weaver, C.,Mao, F.,Kimelman, D.,Xu, W.
Crystal structure of a beta-catenin/Tcf complex.
Cell(Cambridge,Mass.), 103:885-896, 2000

PubMed Abstract: The Wnt signaling pathway plays critical roles in embryonic development and tumorigenesis. Stimulation of the Wnt pathway results in the accumulation of a nuclear beta-catenin/Tcf complex, activating Wnt target genes. A crystal structure of beta-catenin bound to the beta-catenin binding domain of Tcf3 (Tcf3-CBD) has been determined. The Tcf3-CBD forms an elongated structure with three binding modules that runs antiparallel to beta-catenin along the positively charged groove formed by the armadillo repeats. Structure-based mutagenesis defines three sites in beta-catenin that are critical for binding the Tcf3-CBD and are differentially involved in binding APC, cadherin, and Axin. The structural and mutagenesis data reveal a potential target for molecular drug design studies.

PubMed: 11136974
DOI: 10.1016/S0092-8674(00)00192-6

実験手法

X-RAY DIFFRACTION (2.1 Å)