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1G2H

SOLUTION STRUCTURE OF THE DNA-BINDING DOMAIN OF THE TYRR PROTEIN OF HAEMOPHILUS INFLUENZAE

1G2H の概要
エントリーDOI10.2210/pdb1g2h/pdb
分子名称TRANSCRIPTIONAL REGULATORY PROTEIN TYRR HOMOLOG (1 entity in total)
機能のキーワードtyrr; protein structure; nmr; dna-binding domain; helix-turn-helix motif, transcription
由来する生物種Haemophilus influenzae
細胞内の位置Cytoplasm: P44694
タンパク質・核酸の鎖数1
化学式量合計6886.88
構造登録者
Wang, Y.,Zhao, S.,Somerville, R.L.,Jardetzky, O. (登録日: 2000-10-19, 公開日: 2001-03-07, 最終更新日: 2024-05-22)
主引用文献Wang, Y.,Zhao, S.,Somerville, R.L.,Jardetzky, O.
Solution structure of the DNA-binding domain of the TyrR protein of Haemophilus influenzae.
Protein Sci., 10:592-598, 2001
Cited by
PubMed Abstract: The TyrR protein of Haemophilus influenzae is a 36-kD transcription factor whose major function is to control the expression of genes important in the biosynthesis and transport of aromatic amino acids. Using (1)H and (15)N NMR spectroscopy, we have determined the 3D solution structure of the TyrR C-terminal DNA-binding domain (DBD) containing residues from 258 to 318 (TyrR[258-318]). The NMR results show that this segment of TyrR consists of a potential hinge helix at its N terminus (residues 263-270) as well as three well-defined alpha-helices extending from residues 277-289 (HR-2), 293-300 (HR-1), and 304-314 (HR). Helix HR-1 and HR fold in a typical helix-turn-helix (HTH) motif. The three helices and the hinge helix are tightly bound together by hydrophobic interaction and hydrogen bonds. Several hydrophilic residues whose side chains may directly interact with DNA are identified. A hydrophobic patch that may be part of the interaction surface between the domains of TyrR protein is also observed. Comparisons with the structures of other HTH DNA-binding proteins reveal that in terms of the spatial orientation of the three helices, this protein most closely resembles the cap family.
PubMed: 11344327
DOI: 10.1110/ps.45301
主引用文献が同じPDBエントリー
実験手法
SOLUTION NMR
構造検証レポート
Validation report summary of 1g2h
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-16に公開中

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