1FYO

EUKARYOTIC DECODING REGION A-SITE RNA

1FYO の概要

• エントリーDOI: 10.2210/pdb1fyo/pdb
• 関連するPDBエントリー: 1A3M 1FYP 1PBR
• 分子名称: FRAGMENT OF 18S RIBOSOMAL RNA (1 entity in total)
• 機能のキーワード: stem-internal loop-stem-tetraloop rna, g-a base pair, bulged a, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8672.18

構造登録者

Lynch, S.R.,Puglisi, J.D. (登録日: 2000-10-02, 公開日: 2001-03-14, 最終更新日: 2024-05-22)

主引用文献

Lynch, S.R.,Puglisi, J.D.
Structure of a eukaryotic decoding region A-site RNA.
J.Mol.Biol., 306:1023-1035, 2001

PubMed Abstract: The aminoglycoside antibiotics target a region of highly conserved nucleotides in the aminoacyl-tRNA site (A site) of 16 S RNA on the 30 S subunit. The structures of a prokaryotic decoding region A-site oligonucleotide free in solution and bound to the aminoglycosides paromomycin and gentamicin C1A have been determined. Here, the structure of a eukaryotic decoding region A-site oligonucleotide has been determined using homonuclear and heteronuclear NMR spectroscopy, and compared to the unbound prokaryotic rRNA structure. The two structures are similar, with a U1406-U1495 base-pair, a C1407-G1494 Watson-Crick base-pair, and a G1408-A1493 base-pair instead of the A1408-A1493 base-pair of the prokaryotic structure. The two structures differ in the orientation of the 1408 position with respect to A1493; G1408 is rotated toward the major groove, which is the binding pocket for aminoglycosides. The structures also differ in the stacking geometry of G1494 on A1493, which could have slight long-range conformational effects.

PubMed: 11237616
DOI: 10.1006/jmbi.2000.4419

実験手法

SOLUTION NMR