1FXD

REFINED CRYSTAL STRUCTURE OF FERREDOXIN II FROM DESULFOVIBRIO GIGAS AT 1.7 ANGSTROMS

Summary for 1FXD

• Entry DOI: 10.2210/pdb1fxd/pdb
• Descriptor: FERREDOXIN II, FE3-S4 CLUSTER (3 entities in total)
• Functional Keywords: electron transfer(iron-sulfur)
• Biological source: Desulfovibrio gigas
• Total number of polymer chains: 1
• Total formula weight: 6605.81

Authors

Kissinger, C.R.,Sieker, L.C.,Adman, E.T.,Jensen, L.H. (deposition date: 1991-04-08, release date: 1993-04-15, Last modification date: 2025-03-26)

Primary citation

Kissinger, C.R.,Sieker, L.C.,Adman, E.T.,Jensen, L.H.
Refined crystal structure of ferredoxin II from Desulfovibrio gigas at 1.7 A.
J.Mol.Biol., 219:693-715, 1991

PubMed Abstract: The crystal structure of ferredoxin II from Desulfovibrio gigas has been determined using phasing from anomalous scattering data at a resolution of 1.7 A and refined to an R-factor of 0.157. The molecule has an overall chain fold similar to that of the other bacterial ferredoxins of known structure. The molecule contains a single 3Fe-4S cluster with geometry indistinguishable from the 4Fe-4S clusters, and a disulfide bond near the site corresponding to the position of the second cluster of two-cluster ferredoxins. The cluster is bound by cysteine residues 8, 14 and 50. The side-chain of cysteine 11 extends away from the cluster, but could rotate to become the fourth cysteine ligand in the four-iron form of the molecule given a local adjustment of the polypeptide chain. This residue is modified, however, by what appears to be a methanethiol group. There are a total of eight NH . . . S bonds to the inorganic and cysteine sulfur atoms of the Fe-S cluster. There is an additional residue found that is not reported for the chemical sequence: according to the electron density a valine residue should be inserted after residue 55.

PubMed: 2056535
DOI: 10.1016/0022-2836(91)90665-S

Experimental method

X-RAY DIFFRACTION (1.7 Å)