1FXD

REFINED CRYSTAL STRUCTURE OF FERREDOXIN II FROM DESULFOVIBRIO GIGAS AT 1.7 ANGSTROMS

1FXD の概要

• エントリーDOI: 10.2210/pdb1fxd/pdb
• 分子名称: FERREDOXIN II, FE3-S4 CLUSTER (3 entities in total)
• 機能のキーワード: electron transfer(iron-sulfur)
• 由来する生物種: Desulfovibrio gigas
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6605.81

構造登録者

Kissinger, C.R.,Sieker, L.C.,Adman, E.T.,Jensen, L.H. (登録日: 1991-04-08, 公開日: 1993-04-15, 最終更新日: 2025-03-26)

主引用文献

Kissinger, C.R.,Sieker, L.C.,Adman, E.T.,Jensen, L.H.
Refined crystal structure of ferredoxin II from Desulfovibrio gigas at 1.7 A.
J.Mol.Biol., 219:693-715, 1991

PubMed Abstract: The crystal structure of ferredoxin II from Desulfovibrio gigas has been determined using phasing from anomalous scattering data at a resolution of 1.7 A and refined to an R-factor of 0.157. The molecule has an overall chain fold similar to that of the other bacterial ferredoxins of known structure. The molecule contains a single 3Fe-4S cluster with geometry indistinguishable from the 4Fe-4S clusters, and a disulfide bond near the site corresponding to the position of the second cluster of two-cluster ferredoxins. The cluster is bound by cysteine residues 8, 14 and 50. The side-chain of cysteine 11 extends away from the cluster, but could rotate to become the fourth cysteine ligand in the four-iron form of the molecule given a local adjustment of the polypeptide chain. This residue is modified, however, by what appears to be a methanethiol group. There are a total of eight NH . . . S bonds to the inorganic and cysteine sulfur atoms of the Fe-S cluster. There is an additional residue found that is not reported for the chemical sequence: according to the electron density a valine residue should be inserted after residue 55.

PubMed: 2056535
DOI: 10.1016/0022-2836(91)90665-S

実験手法

X-RAY DIFFRACTION (1.7 Å)