1FW6

CRYSTAL STRUCTURE OF A TAQ MUTS-DNA-ADP TERNARY COMPLEX

1FW6 の概要

• エントリーDOI: 10.2210/pdb1fw6/pdb
• 関連するPDBエントリー: 1ewq 1ewr
• 分子名称: 5'-D(*GP*CP*GP*AP*CP*GP*CP*TP*AP*GP*CP*GP*TP*GP*CP*GP*GP*CP*TP*CP*GP*TP*C)-3', 5'-D(*GP*GP*AP*CP*GP*AP*GP*CP*CP*GP*CP*CP*GP*CP*TP*AP*GP*CP*GP*TP*CP*G)-3', DNA MISMATCH REPAIR PROTEIN MUTS, ... (7 entities in total)
• 機能のキーワード: protein-dna complex, multi-domain structure, kinked dna, abc atpase, replication-dna complex, replication/dna
• 由来する生物種: Thermus aquaticus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 188600.14

構造登録者

Junop, M.S.,Obmolova, G.,Rausch, K.,Hsieh, P.,Yang, W. (登録日: 2000-09-21, 公開日: 2001-02-19, 最終更新日: 2024-11-13)

主引用文献

Junop, M.S.,Obmolova, G.,Rausch, K.,Hsieh, P.,Yang, W.
Composite active site of an ABC ATPase: MutS uses ATP to verify mismatch recognition and authorize DNA repair.
Mol.Cell, 7:1-12, 2001

PubMed Abstract: The MutS protein initiates DNA mismatch repair by recognizing mispaired and unpaired bases embedded in duplex DNA and activating endo- and exonucleases to remove the mismatch. Members of the MutS family also possess a conserved ATPase activity that belongs to the ATP binding cassette (ABC) superfamily. Here we report the crystal structure of a ternary complex of MutS-DNA-ADP and assays of initiation of mismatch repair in conjunction with perturbation of the composite ATPase active site by mutagenesis. These studies indicate that MutS has to bind both ATP and the mismatch DNA simultaneously in order to activate the other mismatch repair proteins. We propose that the MutS ATPase activity plays a proofreading role in DNA mismatch repair, verification of mismatch recognition, and authorization of repair.

PubMed: 11172706
DOI: 10.1016/S1097-2765(01)00149-6

実験手法

X-RAY DIFFRACTION (2.7 Å)