1FVA

CRYSTAL STRUCTURE OF BOVINE METHIONINE SULFOXIDE REDUCTASE

1FVA の概要

• エントリーDOI: 10.2210/pdb1fva/pdb
• 関連するPDBエントリー: 1FVG
• 分子名称: PEPTIDE METHIONINE SULFOXIDE REDUCTASE (2 entities in total)
• 機能のキーワード: oxidoreductase
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48065.81

構造登録者

Lowther, W.T.,Brot, N.,Weissbach, H.,Matthews, B.W. (登録日: 2000-09-19, 公開日: 2000-11-08, 最終更新日: 2023-08-09)

主引用文献

Lowther, W.T.,Brot, N.,Weissbach, H.,Matthews, B.W.
Structure and mechanism of peptide methionine sulfoxide reductase, an "anti-oxidation" enzyme.
Biochemistry, 39:13307-13312, 2000

PubMed Abstract: Peptide methionine sulfoxide reductase (MsrA) reverses oxidative damage to both free methionine and methionine within proteins. As such, it helps protect the host organism against stochastic damage that can contribute to cell death. The structure of bovine MsrA has been determined in two different modifications, both of which provide different insights into the biology of the protein. There are three cysteine residues located in the vicinity of the active site. Conformational changes in a glycine-rich C-terminal tail appear to allow all three thiols to come together and to participate in catalysis. The structures support a unique, thiol-disulfide exchange mechanism that relies upon an essential cysteine as a nucleophile and additional conserved residues that interact with the oxygen atom of the sulfoxide moiety.

PubMed: 11063566
DOI: 10.1021/bi0020269

実験手法

X-RAY DIFFRACTION (1.7 Å)