1FV7

A TWO B-Z JUNCTION CONTAINING DNA RESOLVES INTO AN ALL RIGHT HANDED DOUBLE HELIX

1FV7 の概要

• エントリーDOI: 10.2210/pdb1fv7/pdb
• 分子名称: 5'-D(*(5CM)P*GP*(5CM)P*GP*(0DC)P*(0DG)P*(5CM)P*GP*(5CM)P*G)-3' (1 entity in total)
• 機能のキーワード: dna polymorphism, l enantiomery, antisense, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6206.18

構造登録者

Mauffret, O.,El Amri, C.,Santamaria, F.,Tevanian, G.,Rayner, B.,Fermandjian, S. (登録日: 2000-09-19, 公開日: 2000-10-11, 最終更新日: 2024-05-01)

主引用文献

Mauffret, O.,El Amri, C.,Santamaria, F.,Tevanian, G.,Rayner, B.,Fermandjian, S.
A two B-Z junction containing DNA resolves into an all right-handed double-helix.
Nucleic Acids Res., 28:4403-4409, 2000

PubMed Abstract: Natural and artificial oligonucleotides are capable of assuming many different conformations and functions. Here we present results of an NMR restrained molecular modelling study on the conformational preferences of the modified decanucleotide d((m)C1G2(m)C3G4C5(L)G6(L)(m)C7G8(m)C9G10) .d((m)C11G12(m)C13G14C15(L)G (L)16(m)C17-G18(m)C19G20 ) which contains L deoxynucleotides in its centre. This chimeric DNA was expected to form a right-left-right-handed B-type double-helix (BB*B) at low salt concentration. Actually, it matured into a fully right-handed double helix with its central C(L)pG(L) core forming a right-handed Z-DNA helix embedded in a B-DNA matrix (BZ*B). The interplay between base-base and base-sugar stackings within the core and its immediately adjacent residues was found to be critical in ensuring the stabilisation of the right-handed helix. The structure could serve as a model for the design of antisense oligonucleotides resistant to nucleases and capable of hybridising to natural DNAs and RNAs.

PubMed: 11071926
DOI: 10.1093/nar/28.22.4403

実験手法

SOLUTION NMR