1FUX

CRYSTAL STRUCTURE OF E.COLI YBCL, A NEW MEMBER OF THE MAMMALIAN PEBP FAMILY

1FUX の概要

• エントリーDOI: 10.2210/pdb1fux/pdb
• 関連するPDBエントリー: 1A44 1B7A 1BD9 1BEH 1FJJ 1QOU
• 分子名称: HYPOTHETICAL 19.5 KDA PROTEIN IN EMRE-RUS INTERGENIC REGION (2 entities in total)
• 機能のキーワード: beta protein, unknown function
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35633.29

構造登録者

Serre, L.,Pereira de Jesus, K.,Benedetti, H.,Bureaud, N.,Schoentgen, F.,Zelwer, C. (登録日: 2000-09-18, 公開日: 2001-07-18, 最終更新日: 2024-10-30)

主引用文献

Serre, L.,Pereira de Jesus, K.,Zelwer, C.,Bureaud, N.,Schoentgen, F.,Benedetti, H.
Crystal structures of YBHB and YBCL from Escherichia coli, two bacterial homologues to a Raf kinase inhibitor protein.
J.Mol.Biol., 310:617-634, 2001

PubMed Abstract: In rat and human cells, RKIP (previously known as PEBP) was characterized as an inhibitor of the MEK phosphorylation by Raf-1. In Escherichia coli, the genes ybhb and ybcl possibly encode two RKIP homologues while in the genomes of other bacteria and archaebacteria other homologous genes of RKIP have been found. The parallel between the cellular signaling mechanisms in eukaryotes and prokaryotes suggests that these bacterial proteins could be involved in the regulation of protein phosphorylation by kinases as well. We first showed that the proteins YBHB and YBCL were present in the cytoplasm and periplasm of E. coli, respectively, after which we determined their crystallographic structures. These structures verify that YBHB and YBCL belong to the same structural family as mammalian RKIP/PEBP proteins. The general fold and the anion binding site of these proteins are extremely well conserved between mammals and bacteria and suggest functional similarities. However, the bacterial proteins also exhibit some specific structural features, like a substrate binding pocket formed by the dimerization interface and the absence of cis peptide bonds. This structural variety should correspond to the recognition of multiple cellular partners.

PubMed: 11439028
DOI: 10.1006/jmbi.2001.4784

実験手法

X-RAY DIFFRACTION (1.81 Å)