1FTP

THREE-DIMENSIONAL STRUCTURE OF THE MUSCLE FATTY-ACID-BINDING PROTEIN ISOLATED FROM THE DESERT LOCUST, SCHISTOCERCA GREGARIA

1FTP の概要

• エントリーDOI: 10.2210/pdb1ftp/pdb
• 分子名称: MUSCLE FATTY ACID BINDING PROTEIN (2 entities in total)
• 機能のキーワード: binding protein(fatty acid)
• 由来する生物種: Schistocerca gregaria
• 細胞内の位置: Cytoplasm: P41496
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29944.37

構造登録者

Holden, H.M.,Haunerland, N.H. (登録日: 1994-07-06, 公開日: 1994-12-20, 最終更新日: 2024-02-07)

主引用文献

Haunerland, N.H.,Jacobson, B.L.,Wesenberg, G.,Rayment, I.,Holden, H.M.
Three-dimensional structure of the muscle fatty-acid-binding protein isolated from the desert locust Schistocerca gregaria.
Biochemistry, 33:12378-12385, 1994

PubMed Abstract: The three-dimensional structure of the fatty-acid-binding protein isolated from the flight muscle of the desert locust Schistocerca gregaria has been solved and refined to a crystallographic R-value of 18.5% for all measured X-ray data from 30.0- to 2.2-A resolution. Crystals employed in the investigation were grown from 2.6 to 2.8 M ammonium sulfate solutions, buffered at pH 7.5 and containing 2-5% 2-methyl-2,4-pentanediol. They belonged to the space group P2(1) with unit cell dimensions of a = 61.6 A, b = 44.8 A, c = 63.9 A, and beta = 113.6 degrees and two molecules per asymmetric unit. The protein fold consists of ten strands of antiparallel beta-pleated sheet that wrap around to form a beta-barrel. In addition, there are two small alpha-helices and six type I, two type II, and two type II' turns. The two molecules pack in the asymmetric unit as a dimer with a local 2-fold rotational axis. The subunit-subunit interface involves amino acid side chains located in the area of the helix-turn-helix motif and the turn between beta-strands E and F. It is this area that has been speculated to form the portal through which fatty acids enter the binding cavity. There are 23 solvent molecules that are conserved between the two independent molecules in the asymmetric unit. Nine of these waters play important structural roles. A three-dimensional comparison between the insect and human muscle fatty-acid-binding proteins shows that their alpha-carbons superimpose with a root-mean-square deviation of 0.77 A for 89 structurally equivalent atoms.(ABSTRACT TRUNCATED AT 250 WORDS)

PubMed: 7918460
DOI: 10.1021/bi00207a004

実験手法

X-RAY DIFFRACTION (2.2 Å)