1FSL

FERRIC SOYBEAN LEGHEMOGLOBIN COMPLEXED WITH NICOTINATE

1FSL の概要

• エントリーDOI: 10.2210/pdb1fsl/pdb
• 分子名称: LEGHEMOGLOBIN A, PROTOPORPHYRIN IX CONTAINING FE, NICOTINIC ACID, ... (4 entities in total)
• 機能のキーワード: heme, respiratory protein, nitrogen fixation, multigene family, oxygen transport
• 由来する生物種: Glycine max (soybean)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31999.86

構造登録者

Ellis, P.J.,Guss, J.M.,Freeman, H.C. (登録日: 1995-12-12, 公開日: 1996-06-26, 最終更新日: 2024-02-07)

主引用文献

Ellis, P.J.,Appleby, C.A.,Guss, J.M.,Hunter, W.N.,Ollis, D.L.,Freeman, H.C.
Structure of ferric soybean leghemoglobin a nicotinate at 2.3 A resolution.
Acta Crystallogr.,Sect.D, 53:302-310, 1997

PubMed Abstract: Soybean leghemoglobin a is a small (16 kDa) protein facilitating the transport of O(2) to respiring N(2)-fixing bacteria at low free-O(2) tension. The crystal structure of soybean ferric leghemoglobin a nicotinate has been refined at 2.3 A resolution. The final R factor is 15.8% for 6877 reflections between 6.0 and 2.3 A. The structure of soybean leghemoglobin a (143 residues) is closely similar to that of lupin leghemoglobin II (153 residues), the proteins having 82 identical residues when the sequences are aligned. The new structure provides support for the conclusion that the unique properties of leghemoglobin arise principally from a heme pocket considerably larger and more flexible than that of myoglobin, a strongly ruffled heme group, and a proximal histidine orientation more favourable to ligand binding.

PubMed: 15299933
DOI: 10.1107/S0907444997000292

実験手法

X-RAY DIFFRACTION (2.3 Å)