1FPV

STRUCTURE DETERMINATION OF FELINE PANLEUKOPENIA VIRUS EMPTY PARTICLES

Summary for 1FPV

• Entry DOI: 10.2210/pdb1fpv/pdb
• Descriptor: FELINE PANLEUKOPENIA VIRUS (STRAIN B) VIRAL PROTEIN 2 (1 entity in total)
• Functional Keywords: panleukemia virus coat protein, icosahedral virus, virus
• Biological source: Feline panleukopenia virus
• Cellular location: Virion (By similarity): P24840
• Total number of polymer chains: 1
• Total formula weight: 64763.70

Authors

Agbandje, M.,Rossmann, M.G. (deposition date: 1993-03-04, release date: 1994-01-31, Last modification date: 2024-10-23)

Primary citation

Agbandje, M.,McKenna, R.,Rossmann, M.G.,Strassheim, M.L.,Parrish, C.R.
Structure determination of feline panleukopenia virus empty particles.
Proteins, 16:155-171, 1993

PubMed Abstract: Various crystal forms of the single-stranded DNA, feline panleukopenia virus (FPV), a parvovirus, have been grown of both full virions and empty particles. The structure of empty particles crystallized in an orthorhombic space group P2(1)2(1)2(1), with unit cell dimensions a = 380.1 A, b = 379.3 A, and c = 350.9 A, has been determined to 3.3 A resolution. The data were collected using oscillation photography with synchrotron radiation. The orientations of the empty capsids in the unit cell were determined using a self-rotation function and their positions were obtained with an R-factor search using canine parvovirus (CPV) as a model. Phases were then calculated, based on the CPV model, to 6.0 A resolution and gradually extended to 3.3 A resolution by molecular replacement electron density averaging. The resultant electron density was readily interpreted in terms of the known amino acid sequence. The structure is contrasted to that of CPV in terms of host range, neutralization by antibodies, hemagglutination properties, and binding of genomic DNA.

PubMed: 8392729
DOI: 10.1002/prot.340160204

Experimental method

X-RAY DIFFRACTION (3.3 Å)