1FP3

CRYSTAL STRUCTURE OF N-ACYL-D-GLUCOSAMINE 2-EPIMERASE FROM PORCINE KIDNEY

1FP3 の概要

• エントリーDOI: 10.2210/pdb1fp3/pdb
• 分子名称: N-ACYL-D-GLUCOSAMINE 2-EPIMERASE (2 entities in total)
• 機能のキーワード: alpha/alpha-barrel, n-acyl-d-glucosamine 2-epimerase, isomerase
• 由来する生物種: Sus scrofa (pig)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 92930.12

構造登録者

Itoh, T.,Mikami, B.,Maru, I.,Ohta, Y.,Hashimoto, W.,Murata, K. (登録日: 2000-08-30, 公開日: 2000-11-22, 最終更新日: 2024-03-13)

主引用文献

Itoh, T.,Mikami, B.,Maru, I.,Ohta, Y.,Hashimoto, W.,Murata, K.
Crystal structure of N-acyl-D-glucosamine 2-epimerase from porcine kidney at 2.0 A resolution.
J.Mol.Biol., 303:733-744, 2000

PubMed Abstract: The X-ray crystallographic structure of N-acyl-d-glucosamine 2-epimerase (AGE) from porcine kidney, which has been identified to be a renin-binding protein (RnBP), was determined by the multiple isomorphous replacement method and refined at 2.0 A resolution with a final R-factor of 16.9 % for 15 to 2.0 A resolution data. The refined structure of AGE comprised 804 amino acid residues (one dimer) and 145 water molecules. The dimer of AGE had an asymmetric unit with approximate dimensions 46 Ax48 Ax96 A. The AGE monomer is composed of an alpha(6)/alpha(6)-barrel, the structure of which is found in glucoamylase and cellulase. One side of the AGE alpha(6)/alpha(6)-barrel structure comprises long loops containing five short beta-sheets, and contributes to the formation of a deep cleft shaped like a funnel. The putative active-site pocket and a possible binding site for the substrate N-acetyl-d-glucosamine (GlcNAc) were found in the cleft. The other side of the alpha(6)/alpha(6)-barrel comprises short loops and contributes to the dimer formation. At the dimer interface, which is composed of the short loops and alpha-helices of the subunits, five strong ion-pair interactions were observed, which play a major role in the dimer assembly. This completely ruled out the previously accepted hypothesis that the formation of the RnBP homodimer and RnBP-renin heterodimer requires the leucine zipper motif present in RnBP.

PubMed: 11061972
DOI: 10.1006/jmbi.2000.4188

実験手法

X-RAY DIFFRACTION (2 Å)