1FOC

Cytochrome C557: improperly folded thermus thermophilus C552

1FOC の概要

• エントリーDOI: 10.2210/pdb1foc/pdb
• 関連するPDBエントリー: 1C52 1DT1
• 分子名称: CYTOCHROME RC557, HEME C (2 entities in total)
• 機能のキーワード: cytochrome c, thermus thermophilus, heme inversion, electron transport
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29768.31

構造登録者

McRee, D.E.,Williams, P.A.,Fee, J.A.,Bren, K.L. (登録日: 2000-08-27, 公開日: 2000-11-08, 最終更新日: 2024-12-25)

主引用文献

McRee, D.E.,Williams, P.A.,Sridhar, V.,Pastuszyn, A.,Bren, K.L.,Chen, Y.,Patel, K.M.,Todaro, T.R.,Sanders, D.,Luna, E.,Fee, J.A.
Recombinant cytochrome rC557 obtained from Escherichia coli cells expressing a truncated Thermus thermophilus cycA gene. Heme inversion in an improperly matured protein
J.Biol.Chem., 276:6537-6544, 2001

PubMed Abstract: Cytochrome rC(557) is an improperly matured, dimeric cytochrome c obtained from expression of the "signal peptide-lacking" Thermus thermophilus cycA gene in the cytoplasm of Escherichia coli. It is characterized by its Q(00) (or alpha-) optical absorption band at 557 nm in the reduced form (Keightley, J. A., Sanders, D., Todaro, T. R., Pastuszyn, A., and Fee, J. A. (1998) J. Biol. Chem. 273, 12006-12016). We report results of a broad ranging, biochemical and spectral characterization of this protein that reveals the presence of a free vinyl group on the porphyrin and a disulfide bond between the protomers and supports His-Met ligation in both valence states of the iron. A 3-A resolution x-ray structure shows that, in comparison with the native protein, the heme moiety is rotated 180 degrees about its alpha,gamma-axis; cysteine 14 has formed a thioether bond with the 2-vinyl of pyrrole ring I instead of the 4-vinyl of pyrrole ring II, as occurs in the native protein; and a cysteine 11 from each protomer has formed an intermolecular disulfide bond. Numerous, minor perturbations exist within the structure of rC(557) in comparison with that of native protein, which result from heme inversion and protein-protein interactions across the dimer interface. The unusual spectral properties of rC(557) are rationalized in terms of this structure.

PubMed: 11069913
DOI: 10.1074/jbc.M008421200

実験手法

X-RAY DIFFRACTION (3 Å)