1FNN

CRYSTAL STRUCTURE OF CDC6P FROM PYROBACULUM AEROPHILUM

1FNN の概要

• エントリーDOI: 10.2210/pdb1fnn/pdb
• 分子名称: CELL DIVISION CONTROL PROTEIN 6, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: cdc6, cdc18, orc1, aaa protein, dna replication initation factor, cell cycle control factor, cell cycle
• 由来する生物種: Pyrobaculum aerophilum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 89428.64

構造登録者

Liu, J.,Smith, C.L.,DeRyckere, D.,DeAngelis, K.,Martin, G.S.,Berger, J.M. (登録日: 2000-08-22, 公開日: 2000-10-04, 最終更新日: 2024-02-07)

主引用文献

Liu, J.,Smith, C.L.,DeRyckere, D.,DeAngelis, K.,Martin, G.S.,Berger, J.M.
Structure and function of Cdc6/Cdc18: implications for origin recognition and checkpoint control.
Mol.Cell, 6:637-648, 2000

PubMed Abstract: Cdc6/Cdc18 is a conserved and essential component of prereplication complexes. The 2.0 A crystal structure of an archaeal Cdc6 ortholog, in conjunction with a mutational analysis of the homologous Cdc18 protein from Schizosaccharomyces pombe, reveals novel aspects of Cdc6/Cdc18 function. Two domains of Cdc6 form an AAA+-type nucleotide binding fold that is observed bound to Mg.ADP. A third domain adopts a winged-helix fold similar to known DNA binding modules. Sequence comparisons show that the winged-helix domain is conserved in Orc1, and mutagenesis data demonstrate that this region of Cdc6/Cdc18 is required for function in vivo. Additional mutational analyses suggest that nucleotide binding and/or hydrolysis by Cdc6/Cdc18 is required not only for progression through S phase, but also for maintenance of checkpoint control during S phase.

PubMed: 11030343
DOI: 10.1016/S1097-2765(00)00062-9

実験手法

X-RAY DIFFRACTION (2 Å)