1FMY

HIGH RESOLUTION SOLUTION STRUCTURE OF THE PROTEIN PART OF CU7 METALLOTHIONEIN

1FMY の概要

• エントリーDOI: 10.2210/pdb1fmy/pdb
• 分子名称: METALLOTHIONEIN (1 entity in total)
• 機能のキーワード: metallothionein, copper, saccharomyces cerevisiae, metal binding protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4275.66

構造登録者

Bertini, I.,Hartmann, H.J.,Klein, T.,Liu, G.,Luchinat, C.,Weser, U. (登録日: 2000-08-18, 公開日: 2000-09-13, 最終更新日: 2024-05-22)

主引用文献

Bertini, I.,Hartmann, H.J.,Klein, T.,Liu, G.,Luchinat, C.,Weser, U.
High resolution solution structure of the protein part of Cu7 metallothionein.
Eur.J.Biochem., 267:1008-1018, 2000

PubMed Abstract: The three-dimensional solution structure of the protein part of Cu7 metallothionein (Cu7MT) of Saccharomyces cerevisiae has been attempted by 1H two-dimensional NMR spectroscopy at 800 MHz. The protein part constitutes 53 amino acids. A total of 1192 NOEs, of which 1048 are meaningful, were used to determine the solution structure of the first 40 residues, the last 13 residues being disordered. A family of 30 structures was generated. Root-mean-square deviation (rmsd) values from the average structure of 0.32 +/- 0.13 A and 0.61 +/- 0.15 A for backbone and all heavy atoms, respectively, were obtained for the residues 2-40. The ten copper-coordinating cysteine sulfurs and the empty spaces around them are well defined. The structure of the protein part is similar but not identical to the available ones of the same holoprotein and of the Ag7 metallothionein, and is qualitatively superior. If the same metal-sulfur connectivities reported in the literature from 1H-109Ag heteronuclear multiple quantum coherence spectroscopy are assumed to hold for the present copper derivative, a peptide structure is obtained which is again similar, but still not identical, within indetermination, to that available. The structure of the copper polymetallic center may well be different from that proposed for the silver derivative, and indeed a number of different arrangements of the seven copper ions are consistent with the present highly refined structure of the protein part.

PubMed: 10672009
DOI: 10.1046/j.1432-1327.2000.01093.x

実験手法

SOLUTION NMR