1FMM

SOLUTION STRUCTURE OF NFGF-1

1FMM の概要

• エントリーDOI: 10.2210/pdb1fmm/pdb
• 分子名称: ACIDIC FIBROBLAST GROWTH FACTOR (1 entity in total)
• 機能のキーワード: growth factor, triple resonance, mitogen, wound healing, hormone-growth factor complex, hormone/growth factor
• 由来する生物種: Notophthalmus viridescens (eastern newt)
• 細胞内の位置: Secreted (By similarity): Q7SIF8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15033.87

構造登録者

Arunkumar, A.I.,Srisailam, S.,Kumar, T.K.S.,Chiu, I.M.,Yu, C. (登録日: 2000-08-18, 公開日: 2001-08-18, 最終更新日: 2024-05-22)

主引用文献

Arunkumar, A.I.,Srisailam, S.,Kumar, T.K.,Kathir, K.M.,Chi, Y.H.,Wang, H.M.,Chang, G.G.,Chiu, I.,Yu, C.
Structure and stability of an acidic fibroblast growth factor from Notophthalmus viridescens.
J.Biol.Chem., 277:46424-46432, 2002

PubMed Abstract: The three-dimensional solution structure of an acidic fibroblast growth factor (nFGF-1) from the newt (Notophthalmus viridescens) is determined using multidimensional NMR techniques. Complete assignment of all the atoms ((1)H, (15)N, and (13)C) has been achieved using a variety of triple resonance experiments. 50 structures were calculated using hybrid distance geometry-dynamical simulated annealing technique with a total of 1359 constraints. The atomic root mean square distribution for the backbone atoms in the structured region is 0.60 A. The secondary structural elements include 12 beta-strands arranged antiparallely into a beta-barrel structure. The protein (nFGF-1) exists in a monomeric state upon binding to the ligand, sucrose octa sulfate (SOS), in a stoichiometric ratio of 1:1. The SOS binding site consists of a dense cluster of positively charged residues located at the C-terminal end of the molecule. The conformational stabilities of nFGF-1 and its structural and functional homologue from the human source (hFGF-1) are drastically different. The differential stabilities of nFGF-1 and hFGF-1 are attributed to the differences in the number of hydrogen bonds and the presence of solvent inaccessible cavities in the two proteins.

PubMed: 12205097
DOI: 10.1074/jbc.M207814200

実験手法

SOLUTION NMR