1FM2

THE 2 ANGSTROM CRYSTAL STRUCTURE OF CEPHALOSPORIN ACYLASE

1FM2 の概要

• エントリーDOI: 10.2210/pdb1fm2/pdb
• 分子名称: GLUTARYL 7-AMINOCEPHALOSPORANIC ACID ACYLASE (3 entities in total)
• 機能のキーワード: cephalosporin acylase, antibiotics, penicillin acylase, n-terminal hydrolase, hydrolase
• 由来する生物種: Brevundimonas diminuta; 詳細
• 細胞内の位置: Periplasm : Q9L5D6 Q9L5D6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 77253.48

構造登録者

Kim, Y.,Yoon, K.H.,Khang, Y.,Turley, S.,Hol, W.G.J. (登録日: 2000-08-15, 公開日: 2001-08-15, 最終更新日: 2024-11-20)

主引用文献

Kim, Y.,Yoon, K.,Khang, Y.,Turley, S.,Hol, W.G.
The 2.0 A crystal structure of cephalosporin acylase.
Structure Fold.Des., 8:1059-1068, 2000

PubMed Abstract: Semisynthetic cephalosporins are primarily synthesized from 7-aminocephalosporanic acid (7-ACA), which is usually obtained by chemical deacylation of cephalosporin C (CPC). The chemical production of 7-ACA includes, however, several expensive steps and requires thorough treatment of chemical wastes. Therefore, an enzymatic conversion of CPC to 7-ACA by cephalosporin acylase is of great interest. The biggest obstacle preventing this in industrial production is that cephalosporin acylase uses glutaryl-7ACA as a primary substrate and has low substrate specificity for CPC.

PubMed: 11080627
DOI: 10.1016/S0969-2126(00)00505-0

実験手法

X-RAY DIFFRACTION (2 Å)