1FL8

HYPERMODIFIED NUCLEOSIDES IN THE ANTICODON OF TRNALYS STABILIZE A CANONICAL U-TURN STRUCTURE

1FL8 の概要

• エントリーDOI: 10.2210/pdb1fl8/pdb
• 関連するPDBエントリー: 1BZ2 1BZU
• 分子名称: ANTICODON DOMAIN OF TRNA(LYS) (1 entity in total)
• 機能のキーワード: trna, anticodon, pseudouridine, mnm5s2u, t6a, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5554.44

構造登録者

Sundaram, M.,Durant, P.C.,Davis, D.R. (登録日: 2000-08-11, 公開日: 2000-10-16, 最終更新日: 2024-05-22)

主引用文献

Sundaram, M.,Durant, P.C.,Davis, D.R.
Hypermodified nucleosides in the anticodon of tRNALys stabilize a canonical U-turn structure.
Biochemistry, 39:12575-12584, 2000

PubMed Abstract: Modified nucleosides in the anticodon domain of Escherichia coli tRNA(Lys) are necessary for high-affinity codon recognition and reading frame maintenance. Human tRNA(Lys,3) is the specific primer for HIV-1 reverse transcriptase and also requires nucleoside modification for proper function. We now present NMR solution structures for the fully modified 17-nucleotide E. coli tRNA(Lys) anticodon stem-loop domain (ASL). NMR data were also collected for several partially modified ASLs, revealing the contributions each modified nucleoside (mnm(5)s(2)U34, t(6)A37, and psi39) makes in transforming the disordered, unmodified tRNA ASL into the highly ordered native structure. The solution structure of the native ASL domain provides insight into longstanding questions regarding both wobble position modification and the nearly ubiquitous t(6)A37 found in tRNAs with an adjacent U at position 36. Native tRNA(Lys) has a U-turn structure similar to the yeast tRNA(Phe) crystal structure, unlike previously proposed "unconventional" anticodon structures characterized by stable interactions between mnm(5)s(2)U-34 and t(6)A-37.

PubMed: 11027137
DOI: 10.1021/bi0014655

実験手法

SOLUTION NMR