1FL2

CATALYTIC CORE COMPONENT OF THE ALKYLHYDROPEROXIDE REDUCTASE AHPF FROM E.COLI

1FL2 の概要

• エントリーDOI: 10.2210/pdb1fl2/pdb
• 分子名称: ALKYL HYDROPEROXIDE REDUCTASE SUBUNIT F, SULFATE ION, FLAVIN-ADENINE DINUCLEOTIDE, ... (4 entities in total)
• 機能のキーワード: alkylhydroperoxide reductase, reactive oxygen, fad, disulphide oxidoreductase, oxidoreductase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34197.40

構造登録者

Bieger, B.,Essen, L.-O. (登録日: 2000-08-11, 公開日: 2001-03-21, 最終更新日: 2024-10-09)

主引用文献

Bieger, B.,Essen, L.O.
Crystal structure of the catalytic core component of the alkylhydroperoxide reductase AhpF from Escherichia coli.
J.Mol.Biol., 307:1-8, 2001

PubMed Abstract: Alkylhydroperoxide reductases (AhpR, EC 1.6.4.*) are essential for the oxygen tolerance of aerobic organisms by converting otherwise toxic hydroperoxides of lipids or nucleic acids to the corresponding alcohols. The AhpF component belongs to the family of pyridine nucleotide-disulphide oxidoreductases and channels electrons from NAD(P)H towards the AhpC component which finally reduces cognate substrates. The structure of the catalytic core of the Escherichia coli AhpF (A212-A521) with a bound FAD cofactor was determined at 1.9 A resolution in its oxidized state. The dimeric arrangement of the AhpF catalytic core and the predicted interaction mode between the N-terminal PDO-like domain and the NADPH domain favours an intramolecular electron transfer between the two redox-active disulphide centres of AhpF.

PubMed: 11243797
DOI: 10.1006/jmbi.2000.4441

実験手法

X-RAY DIFFRACTION (1.9 Å)