1FJE

SOLUTION STRUCTURE OF NUCLEOLIN RBD12 IN COMPLEX WITH SNRE RNA

1FJE の概要

• エントリーDOI: 10.2210/pdb1fje/pdb
• 関連するPDBエントリー: 1FJ7 1FJC
• 分子名称: SNRE RNA, NUCLEOLIN RBD12 (2 entities in total)
• 機能のキーワード: rnp, rbd, rrm, rna binding domain, rna-protein complex, nucleolus, structural protein-rna complex, structural protein/rna
• 由来する生物種: Mesocricetus auratus (golden hamster); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26461.07

構造登録者

Allain, F.H.T.,Bouvet, P.,Dieckmann, T.,Feigon, J. (登録日: 2000-08-08, 公開日: 2001-01-03, 最終更新日: 2024-05-22)

主引用文献

Allain, F.H.,Bouvet, P.,Dieckmann, T.,Feigon, J.
Molecular basis of sequence-specific recognition of pre-ribosomal RNA by nucleolin.
EMBO J., 19:6870-6881, 2000

PubMed Abstract: The structure of the 28 kDa complex of the first two RNA binding domains (RBDs) of nucleolin (RBD12) with an RNA stem-loop that includes the nucleolin recognition element UCCCGA in the loop was determined by NMR spectroscopy. The structure of nucleolin RBD12 with the nucleolin recognition element (NRE) reveals that the two RBDs bind on opposite sides of the RNA loop, forming a molecular clamp that brings the 5' and 3' ends of the recognition sequence close together and stabilizing the stem-loop. The specific interactions observed in the structure explain the sequence specificity for the NRE sequence. Binding studies of mutant proteins and analysis of conserved residues support the proposed interactions. The mode of interaction of the protein with the RNA and the location of the putative NRE sites suggest that nucleolin may function as an RNA chaperone to prevent improper folding of the nascent pre-rRNA.

PubMed: 11118222
DOI: 10.1093/emboj/19.24.6870

実験手法

SOLUTION NMR