1FI2

CRYSTAL STRUCTURE OF GERMIN (OXALATE OXIDASE)

1FI2 の概要

• エントリーDOI: 10.2210/pdb1fi2/pdb
• 関連するPDBエントリー: 2PHL
• 分子名称: OXALATE OXIDASE, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: beta-jellyroll, oxidoreductase
• 由来する生物種: Hordeum vulgare
• 細胞内の位置: Secreted, extracellular space, apoplast (By similarity): P45850
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21292.11

構造登録者

Woo, E.J.,Dunwell, J.M.,Goodenough, P.W.,Marvier, A.C.,Pickersgill, R.W. (登録日: 2000-08-03, 公開日: 2001-05-01, 最終更新日: 2024-11-13)

主引用文献

Woo, E.J.,Dunwell, J.M.,Goodenough, P.W.,Marvier, A.C.,Pickersgill, R.W.
Germin is a manganese containing homohexamer with oxalate oxidase and superoxide dismutase activities.
Nat.Struct.Biol., 7:1036-1040, 2000

PubMed Abstract: Germin is a hydrogen peroxide generating oxalate oxidase with extreme thermal stability; it is involved in the defense against biotic and abiotic stress in plants. The structure, determined at 1.6 A resolution, comprises beta-jellyroll monomers locked into a homohexamer (a trimer of dimers), with extensive surface burial accounting for its remarkable stability. The germin dimer is structurally equivalent to the monomer of the 7S seed storage proteins (vicilins), indicating evolution from a common ancestral protein. A single manganese ion is bound per germin monomer by ligands similar to those of manganese superoxide dismutase (MnSOD). Germin is also shown to have SOD activity and we propose that the defense against extracellular superoxide radicals is an important additional role for germin and related proteins.

PubMed: 11062559
DOI: 10.1038/80954

実験手法

X-RAY DIFFRACTION (1.6 Å)