1FHY

PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION

1FHY の概要

• エントリーDOI: 10.2210/pdb1fhy/pdb
• 分子名称: DNA (5'-D(*CP*CP*GP*CP*TP*AP*GP*CP*GP*G)-3'), 4'-HYDROXYMETHYL-4,5',8-TRIMETHYLPSORALEN, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: psoralen, cross-linked dna, holliday junction, 4-way junction, dna-drug complex, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6390.33

構造登録者

Eichman, B.F.,Mooers, B.H.M.,Alberti, M.,Hearst, J.E.,Ho, P.S. (登録日: 2000-08-02, 公開日: 2001-04-21, 最終更新日: 2024-02-07)

主引用文献

Eichman, B.F.,Mooers, B.H.,Alberti, M.,Hearst, J.E.,Ho, P.S.
The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions.
J.Mol.Biol., 308:15-26, 2001

PubMed Abstract: The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.

PubMed: 11302703
DOI: 10.1006/jmbi.2001.4567

実験手法

X-RAY DIFFRACTION (2.2 Å)