1FDN

REFINED CRYSTAL STRUCTURE OF THE 2[4FE-4S] FERREDOXIN FROM CLOSTRIDIUM ACIDURICI AT 1.84 ANGSTROMS RESOLUTION

1FDN の概要

• エントリーDOI: 10.2210/pdb1fdn/pdb
• 分子名称: FERREDOXIN, IRON/SULFUR CLUSTER (3 entities in total)
• 機能のキーワード: electron transport
• 由来する生物種: Clostridium acidurici
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6243.46

構造登録者

Duee, E.,Fanchon, E.,Vicat, J.,Sieker, L.C.,Meyer, J.,Moulis, J-M. (登録日: 1994-03-31, 公開日: 1994-08-31, 最終更新日: 2024-02-07)

主引用文献

Duee, E.D.,Fanchon, E.,Vicat, J.,Sieker, L.C.,Meyer, J.,Moulis, J.M.
Refined crystal structure of the 2[4Fe-4S] ferredoxin from Clostridium acidurici at 1.84 A resolution.
J.Mol.Biol., 243:683-695, 1994

PubMed Abstract: The crystal structure of the 2[4Fe-4S] ferredoxin from Clostridium acidurici has been determined at a resolution of 1.84 A and refined to an R-factor of 0.169. Crystals belong to space group P4(3)2(1)2 with unit cell dimensions a = b = 34.44 A and c = 74.78 A. The structure was determined by molecular replacement using the previously published model of an homologous ferredoxin and refined by molecular dynamics techniques. The model contains the protein and 46 water molecules. Only two amino acid residues, Asp27 and Asp28, are poorly defined in the electron density maps. The molecule has an overall chain fold similar to that of other [4Fe-4S] bacterial ferredoxins of known structure. The two [4Fe-4S] clusters display similar bond distances and angles. In both of them the co-ordination of one iron atom (bound to Cys11 and Cys40) is slightly distorted as compared with that of the other iron atoms. A core of hydrophobic residues and a few water molecules contribute to the stability of the structure. The [4Fe-4S] clusters interact with the polypeptide chain through eight hydrogen bonds each, in addition to the covalent Fe-Scys bonds. The ferredoxin from Clostridium acidurici is the most typical clostridial ferredoxin crystallized so far and the biological implications of the newly determined structure are discussed.

PubMed: 7966291
DOI: 10.1016/0022-2836(94)90041-8

実験手法

X-RAY DIFFRACTION (1.84 Å)