1FCW
TRNA POSITIONS DURING THE ELONGATION CYCLE
Summary for 1FCW
| Entry DOI | 10.2210/pdb1fcw/pdb |
| Related | 486D |
| Descriptor | TRNAPHE (1 entity in total) |
| Functional Keywords | ribosome, trna binding sites, protein synthesis, elongation cycle |
| Biological source | Saccharomyces cerevisiae (baker's yeast) |
| Total number of polymer chains | 5 |
| Total formula weight | 124450.60 |
| Authors | Agrawal, R.K.,Spahn, C.M.T.,Penczek, P.,Grassucci, R.A.,Nierhaus, K.H.,Frank, J. (deposition date: 2000-07-19, release date: 2000-08-11, Last modification date: 2024-02-07) |
| Primary citation | Agrawal, R.K.,Spahn, C.M.,Penczek, P.,Grassucci, R.A.,Nierhaus, K.H.,Frank, J. Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle. J.Cell Biol., 150:447-459, 2000 Cited by PubMed Abstract: Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-A resolution. The positions of tRNAs in the A and P sites in the pretranslocational complexes and in the P and E sites in the posttranslocational complexes have been determined. Of these, the P-site tRNA position is the same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visualized with high accuracy. Now, the positions of the A- and E-site tRNAs are determined with similar accuracy. The positions of the CCA end of the tRNAs at the A site are different before and after peptide bond formation. The relative positions of anticodons of P- and E-site tRNAs in the posttranslocational state are such that a codon-anticodon interaction at the E site appears feasible. PubMed: 10931859DOI: 10.1083/jcb.150.3.447 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (17 Å) |
Structure validation
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