1FCW

TRNA POSITIONS DURING THE ELONGATION CYCLE

1FCW の概要

• エントリーDOI: 10.2210/pdb1fcw/pdb
• 関連するPDBエントリー: 486D
• 分子名称: TRNAPHE (1 entity in total)
• 機能のキーワード: ribosome, trna binding sites, protein synthesis, elongation cycle
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 124450.60

構造登録者

Agrawal, R.K.,Spahn, C.M.T.,Penczek, P.,Grassucci, R.A.,Nierhaus, K.H.,Frank, J. (登録日: 2000-07-19, 公開日: 2000-08-11, 最終更新日: 2024-02-07)

主引用文献

Agrawal, R.K.,Spahn, C.M.,Penczek, P.,Grassucci, R.A.,Nierhaus, K.H.,Frank, J.
Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle.
J.Cell Biol., 150:447-459, 2000

PubMed Abstract: Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-A resolution. The positions of tRNAs in the A and P sites in the pretranslocational complexes and in the P and E sites in the posttranslocational complexes have been determined. Of these, the P-site tRNA position is the same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visualized with high accuracy. Now, the positions of the A- and E-site tRNAs are determined with similar accuracy. The positions of the CCA end of the tRNAs at the A site are different before and after peptide bond formation. The relative positions of anticodons of P- and E-site tRNAs in the posttranslocational state are such that a codon-anticodon interaction at the E site appears feasible.

PubMed: 10931859
DOI: 10.1083/jcb.150.3.447

実験手法

ELECTRON MICROSCOPY (17 Å)