1FAG

STRUCTURE OF CYTOCHROME P450

1FAG の概要

• エントリーDOI: 10.2210/pdb1fag/pdb
• 分子名称: CYTOCHROME P450 BM-3, PROTOPORPHYRIN IX CONTAINING FE, PALMITOLEIC ACID (3 entities in total)
• 機能のキーワード: monooxygenase, electron transport, heme
• 由来する生物種: Bacillus megaterium
• 細胞内の位置: Cytoplasm (By similarity): P14779
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 218668.75

構造登録者

Li, H.Y.,Poulos, T.L. (登録日: 1996-08-01, 公開日: 1997-02-12, 最終更新日: 2024-04-03)

主引用文献

Li, H.,Poulos, T.L.
The structure of the cytochrome p450BM-3 haem domain complexed with the fatty acid substrate, palmitoleic acid.
Nat.Struct.Biol., 4:140-146, 1997

PubMed Abstract: The substrate-bound structures of two cytochrome P450s, P450cam and P450eryF, are known. While these structures reveal important features that control substrate specificity, the problem of how conformational changes allow for substrate entry and product release remains unsolved. The structure of the haem domain of the bacterial fatty acid hydroxylase, P450BM-3, previously was solved in the substrate-free form. Unlike the substrate-bound P450cam and P450eryF structures, the substrate access channel is open in substrate-free P450BM-3. Here we present the X-ray structure of P450BM-3 at 2.7 A bound with a fatty acid substrate, palmitoleic acid. A comparison of the substrate-bound and -free forms reveals major conformational differences and provides the first detailed picture of substrate-induced conformational changes in a P450.

PubMed: 9033595
DOI: 10.1038/nsb0297-140

実験手法

X-RAY DIFFRACTION (2.7 Å)