1F9X

AVERAGE NMR SOLUTION STRUCTURE OF THE BIR-3 DOMAIN OF XIAP

1F9X の概要

• エントリーDOI: 10.2210/pdb1f9x/pdb
• 分子名称: INHIBITOR OF APOPTOSIS PROTEIN XIAP, ZINC ION (2 entities in total)
• 機能のキーワード: bir3 domain, inhibitor of apoptosis protein xiap, zinc finger, caspase-9 inhibition, apoptosis inhibitor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P98170
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13772.64

構造登録者

Sun, C.,Cai, M.,Meadows, R.P.,Fesik, S.W. (登録日: 2000-07-11, 公開日: 2001-07-11, 最終更新日: 2024-05-22)

主引用文献

Sun, C.,Cai, M.,Meadows, R.P.,Xu, N.,Gunasekera, A.H.,Herrmann, J.,Wu, J.C.,Fesik, S.W.
NMR structure and mutagenesis of the third Bir domain of the inhibitor of apoptosis protein XIAP.
J.Biol.Chem., 275:33777-33781, 2000

PubMed Abstract: The inhibitor of apoptosis proteins (IAPs) regulate the caspase family of cysteine proteases, which play an important role in the execution of programmed cell death. Human X-linked inhibitor of apoptosis protein (XIAP) is a potent inhibitor of caspases-3, -7, and -9. Here we show that the Bir3 domain is the minimal region of XIAP that is needed for potent caspase-9 inhibition. The three-dimensional structure of the Bir3 domain of XIAP, determined by NMR spectroscopy, resembles a classical zinc finger and consists of five alpha-helices, a three-stranded beta-sheet, and a zinc atom chelated to three cysteines and one histidine. The structure of the Bir3 domain is similar to that of the Bir2 domain of XIAP but differs from the previously determined structure of the Bir3 domain of MIHB. Based on site-directed mutagenesis, we have identified the regions of the Bir3 domain of XIAP that are important for inhibiting caspase-9. Despite the structural similarities of the Bir2 and Bir3 domain of XIAP, a different set of residues were found to be critical for inhibiting the individual caspases. These results suggest that XIAP inhibits caspase-3 and caspase-9 in a different manner.

PubMed: 10934209
DOI: 10.1074/jbc.M006226200

実験手法

SOLUTION NMR