1F8Z

NMR STRUCTURE OF THE SIXTH LIGAND-BINDING MODULE OF THE LDL RECEPTOR

1F8Z の概要

• エントリーDOI: 10.2210/pdb1f8z/pdb
• 関連するPDBエントリー: 1LDL 1LDR
• 分子名称: LOW-DENSITY LIPOPROTEIN RECEPTOR, CALCIUM ION (2 entities in total)
• 機能のキーワード: ldl receptor, ligand-binding domain, calcium-binding, familial hypercholesterolemia, lipid binding protein
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein: P01130
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4432.84

構造登録者

Clayton, D.J.,Brereton, I.M.,Kroon, P.A.,Smith, R. (登録日: 2000-07-05, 公開日: 2000-10-18, 最終更新日: 2024-10-30)

主引用文献

Clayton, D.,Brereton, I.M.,Kroon, P.A.,Smith, R.
Three-dimensional NMR structure of the sixth ligand-binding module of the human LDL receptor: comparison of two adjacent modules with different ligand binding specificities.
FEBS Lett., 479:118-122, 2000

PubMed Abstract: The sixth ligand-binding module of the low-density lipoprotein receptor contributes to the binding of apolipoprotein B100-containing lipoproteins. 1H NMR spectroscopy, DYANA and X-PLOR structure calculations were used to determine that this module has a well defined structure with a backbone conformation similar to other modules. Structures from calculations that simulated the presence of a calcium ion showed increased resolution without large increases in energy, increased deviations from idealised geometry or violations of experimental constraints. Investigation of the surface properties of this module indicates there are significant differences from the fifth module, which binds apolipoprotein E-containing lipoproteins in addition to apolipoprotein B100-containing lipoproteins.

PubMed: 10981718
DOI: 10.1016/S0014-5793(00)01842-1

実験手法

SOLUTION NMR