1F8V

THE STRUCTURE OF PARIACOTO VIRUS REVEALS A DODECAHEDRAL CAGE OF DUPLEX RNA

1F8V の概要

• エントリーDOI: 10.2210/pdb1f8v/pdb
• 関連するPDBエントリー: 1nov 2bbv
• 分子名称: RNA, MATURE CAPSID PROTEIN BETA, MATURE CAPSID PROTEIN GAMMA, ... (6 entities in total)
• 機能のキーワード: nodavirus, coat protein, nucleoprotein, protein-rna interactions, rna duplex, rna cage, gamma polypeptide, beta-sandwich, icosahedral virus, virus-rna complex, virus/rna
• 由来する生物種: Pariacato virus; 詳細
• 細胞内の位置: Capsid protein beta: Virion . Peptide gamma: Virion : Q9J7Z0 Q9J7Z0
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 135878.88

構造登録者

Tang, L.,Johnson, K.N.,Ball, L.A.,Lin, T.,Yeager, M.,Johnson, J.E. (登録日: 2000-07-05, 公開日: 2000-12-06, 最終更新日: 2024-04-03)

主引用文献

Tang, L.,Johnson, K.N.,Ball, L.A.,Lin, T.,Yeager, M.,Johnson, J.E.
The structure of pariacoto virus reveals a dodecahedral cage of duplex RNA.
Nat.Struct.Biol., 8:77-83, 2001

PubMed Abstract: The 3.0 A resolution crystal structure of Pariacoto virus (PaV) reveals extensive interactions between portions of the viral RNA genome and the icosahedral capsid. Under the protein shell of the T = 3 quasi equivalent capsid lies a dodecahedral cage composed of RNA duplex that accounts for approximately 35% of the single-stranded RNA genome. The highly basic N-terminal regions (residues 7-54) of the subunits, forming pentamers (A subunits) are clearly visible in the density map and make numerous interactions with the RNA cage. The C-terminal segments (residues 394-401) of the A subunits lie in channels near the quasi three-fold axes. Electron cryo-microscopy and image reconstruction of PaV particles clearly show the dodecahedral RNA cage.

PubMed: 11135676
DOI: 10.1038/83089

実験手法

X-RAY DIFFRACTION (3 Å)