1F85

SOLUTION STRUCTURE OF HCV IRES RNA DOMAIN IIIE

Summary for 1F85

• Entry DOI: 10.2210/pdb1f85/pdb
• Descriptor: HCV-1B IRES RNA DOMAIN IIIE (1 entity in total)
• Functional Keywords: ribonucleic acid, hepatitis c virus internal ribosome entry site, hairpin loop, tetraloop, rna structure, rna
• Total number of polymer chains: 1
• Total formula weight: 4518.73

Authors

Lukavsky, P.J.,Otto, G.A.,Lancaster, A.M.,Sarnow, P.,Puglisi, J.D. (deposition date: 2000-06-28, release date: 2000-11-17, Last modification date: 2024-05-22)

Primary citation

Lukavsky, P.J.,Otto, G.A.,Lancaster, A.M.,Sarnow, P.,Puglisi, J.D.
Structures of two RNA domains essential for hepatitis C virus internal ribosome entry site function.
Nat.Struct.Biol., 7:1105-1110, 2000

PubMed Abstract: Translation of the hepatitis C virus (HCV) polyprotein is initiated at an internal ribosome entry site (IRES) element in the 5' untranslated region of HCV RNA. The HCV IRES element interacts directly with the 40S subunit, and biochemical experiments have implicated RNA elements near the AUG start codon as required for IRES-40S subunit complex formation. The data we present here show that two RNA stem loops, domains IIId and IIIe, are involved in IRES-40S subunit interaction. The structures of the two RNA domains were solved by NMR spectroscopy and reveal structural features that may explain their role in IRES function.

PubMed: 11101890
DOI: 10.1038/81951

Experimental method

SOLUTION NMR