1F84

SOLUTION STRUCTURE OF HCV IRES RNA DOMAIN IIID

Summary for 1F84

Entry DOI	10.2210/pdb1f84/pdb
Descriptor	HCV-1B IRES RNA DOMAIN IIID (1 entity in total)
Functional Keywords	ribonucleic acid, hepatitis c virus internal ribosome entry site, sarcin/ricin loop, loop e motif, hairpin loop, rna structure, rna
Total number of polymer chains	1
Total formula weight	9450.67
Authors	Lukavsky, P.J.,Otto, G.A.,Lancaster, A.M.,Sarnow, P.,Puglisi, J.D. (deposition date: 2000-06-28, release date: 2000-11-17, Last modification date: 2024-05-22)
Primary citation	Lukavsky, P.J.,Otto, G.A.,Lancaster, A.M.,Sarnow, P.,Puglisi, J.D. Structures of two RNA domains essential for hepatitis C virus internal ribosome entry site function. Nat.Struct.Biol., 7:1105-1110, 2000 Cited by PubMed Abstract: Translation of the hepatitis C virus (HCV) polyprotein is initiated at an internal ribosome entry site (IRES) element in the 5' untranslated region of HCV RNA. The HCV IRES element interacts directly with the 40S subunit, and biochemical experiments have implicated RNA elements near the AUG start codon as required for IRES-40S subunit complex formation. The data we present here show that two RNA stem loops, domains IIId and IIIe, are involved in IRES-40S subunit interaction. The structures of the two RNA domains were solved by NMR spectroscopy and reveal structural features that may explain their role in IRES function. PubMed: 11101890 DOI: 10.1038/81951 PDB entries with the same primary citation
Experimental method	SOLUTION NMR

Structure validation