1F84

SOLUTION STRUCTURE OF HCV IRES RNA DOMAIN IIID

1F84 の概要

• エントリーDOI: 10.2210/pdb1f84/pdb
• 分子名称: HCV-1B IRES RNA DOMAIN IIID (1 entity in total)
• 機能のキーワード: ribonucleic acid, hepatitis c virus internal ribosome entry site, sarcin/ricin loop, loop e motif, hairpin loop, rna structure, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9450.67

構造登録者

Lukavsky, P.J.,Otto, G.A.,Lancaster, A.M.,Sarnow, P.,Puglisi, J.D. (登録日: 2000-06-28, 公開日: 2000-11-17, 最終更新日: 2024-05-22)

主引用文献

Lukavsky, P.J.,Otto, G.A.,Lancaster, A.M.,Sarnow, P.,Puglisi, J.D.
Structures of two RNA domains essential for hepatitis C virus internal ribosome entry site function.
Nat.Struct.Biol., 7:1105-1110, 2000

PubMed Abstract: Translation of the hepatitis C virus (HCV) polyprotein is initiated at an internal ribosome entry site (IRES) element in the 5' untranslated region of HCV RNA. The HCV IRES element interacts directly with the 40S subunit, and biochemical experiments have implicated RNA elements near the AUG start codon as required for IRES-40S subunit complex formation. The data we present here show that two RNA stem loops, domains IIId and IIIe, are involved in IRES-40S subunit interaction. The structures of the two RNA domains were solved by NMR spectroscopy and reveal structural features that may explain their role in IRES function.

PubMed: 11101890
DOI: 10.1038/81951

実験手法

SOLUTION NMR