1F7P

CRYSTAL STRUCTURES OF FELINE IMMUNODEFICIENCY VIRUS DUTP PYROPHOSPHATASE AND ITS NUCLEOTIDE COMPLEXES IN THREE CRYSTAL FORMS.

1F7P の概要

• エントリーDOI: 10.2210/pdb1f7p/pdb
• 関連するPDBエントリー: 1F7D 1F7K 1F7N 1F7O 1F7Q 1F7R
• 分子名称: POL POLYPROTEIN, URIDINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: eight stranded beta barrel protein, viral protein, hydrolase
• 由来する生物種: Feline immunodeficiency virus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 44998.83

構造登録者

Prasad, G.S.,Stura, E.A.,Elder, J.H.,Stout, C.D. (登録日: 2000-06-27, 公開日: 2000-09-06, 最終更新日: 2024-02-07)

主引用文献

Prasad, G.S.,Stura, E.A.,Elder, J.H.,Stout, C.D.
Structures of feline immunodeficiency virus dUTP pyrophosphatase and its nucleotide complexes in three crystal forms.
Acta Crystallogr.,Sect.D, 56:1100-1109, 2000

PubMed Abstract: dUTP pyrophosphatase (dUTPase) cleaves the alpha-beta phosphodiester of dUTP to form pyrophosphate and dUMP, preventing incorporation of uracil into DNA and providing the substrate for thymine synthesis. Seven crystal structures of feline immunodeficiency virus (FIV) dUTPase in three crystal forms have been determined, including complexes with substrate (dUTP), product (dUMP) or inhibitor (dUDP) bound. The native enzyme has been refined at 1.40 A resolution in a hexagonal crystal form and at 2.3 A resolution in an orthorhombic crystal form. In the dUDP complex in a cubic crystal form refined at 2.5 A resolution, the C-terminal conserved P-loop motif is fully ordered. The analysis defines the roles of five sequence motifs in interaction with uracil, deoxyribose and the alpha-, beta- and gamma-phosphates. The enzyme utilizes adaptive recognition to bind the alpha- and beta-phosphates. In particular, the alpha-beta phosphodiester adopts an unfavorable eclipsed conformation in the presence of the P-loop. This conformation may be relevant to the mechanism of alpha-beta phosphodiester bond cleavage.

PubMed: 10957629
DOI: 10.1107/S0907444900009197

実験手法

X-RAY DIFFRACTION (2.3 Å)