1F7G

SOLUTION STRUCTURE OF THE RNASE P RNA (M1 RNA) P4 STEM C70U MUTANT OLIGORIBONUCLEOTIDE, ENSEMBLE OF 17 STRUCTURES

1F7G の概要

• エントリーDOI: 10.2210/pdb1f7g/pdb
• 関連するPDBエントリー: 1F6Z
• 分子名称: RNASE P RNA RIBOZYME, P4 DOMAIN (1 entity in total)
• 機能のキーワード: ribonuclease p, ribozyme, transfer rna processing, p4 stem, c70u mutant, metal binding site, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8634.13

構造登録者

Schmitz, M.,Tinoco Jr., I. (登録日: 2000-06-27, 公開日: 2000-10-09, 最終更新日: 2024-05-22)

主引用文献

Schmitz, M.,Tinoco Jr., I.
Solution structure and metal-ion binding of the P4 element from bacterial RNase P RNA.
RNA, 6:1212-1225, 2000

PubMed Abstract: We determined the solution structure of two 27-nt RNA hairpins and their complexes with cobalt(III)-hexammine (Co(NH3)3+(6)) by NMR spectroscopy. The RNA hairpins used in this study are the P4 region from Escherichia coli RNase P RNA and a C-to-U mutant that confers altered divalent metal-ion specificity (Ca2+ replaces Mg2+) for catalytic activity of this ribozyme. Co(NH3)3+(6) is a useful spectroscopic probe for Mg(H2O)2+(6)-binding sites because both complexes have octahedral symmetry and have similar radii. The thermodynamics of binding to both RNA hairpins was studied using chemical shift changes upon titration with Mg2+, Ca2+, and Co(NH3)3+(6). We found that the equilibrium binding constants for each of the metal ions was essentially unchanged when the P4 model RNA hairpin was mutated, although the NMR structures show that the RNA hairpins adopt different conformations. In the C-to-U mutant a C.G base pair is replaced by U.G, and the conserved bulged uridine in the P4 wild-type stem shifts in the 3' direction by 1 nt. Intermolecular NOE cross-peaks between Co(NH3)3+(6) and RNA protons were used to locate the site of Co(NH3)3+(6) binding to both RNA hairpins. The metal ion binds in the major groove near a bulge loop, but is shifted 5' by more than 1 bp in the mutant. The change of the metal-ion binding site provides a possible explanation for changes in catalytic activity of the mutant RNase P in the presence of Ca2+.

PubMed: 10999599
DOI: 10.1017/S1355838200000881

実験手法

SOLUTION NMR