1F6V

SOLUTION STRUCTURE OF THE C TERMINAL OF MU B TRANSPOSITION PROTEIN

1F6V の概要

• エントリーDOI: 10.2210/pdb1f6v/pdb
• 分子名称: DNA TRANSPOSITION PROTEIN (1 entity in total)
• 機能のキーワード: mu phage, recombination, transposition, atpase, dna binding, high salt, solution structure, dna binding protein
• 由来する生物種: Enterobacteria phage Mu
• 細胞内の位置: Host cytoplasm : P03763
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10237.76

構造登録者

Hung, L.-H.,Chaconas, G.,Shaw, G.S. (登録日: 2000-06-23, 公開日: 2000-11-08, 最終更新日: 2024-05-01)

主引用文献

Hung, L.H.,Chaconas, G.,Shaw, G.S.
The solution structure of the C-terminal domain of the Mu B transposition protein.
EMBO J., 19:5625-5634, 2000

PubMed Abstract: Mu B is one of four proteins required for the strand transfer step of bacteriophage Mu DNA transposition and the only one where no high resolution structural data is available. Structural work on Mu B has been hampered primarily by solubility problems and its tendency to aggregate. We have overcome this problem by determination of the three-dimensional structure of the C-terminal domain of Mu B (B(223-312)) in 1.5 M NaCl using NMR spectroscopic methods. The structure of Mu B(223-312) comprises four helices (backbone r.m.s.d. 0.46 A) arranged in a loosely packed bundle and resembles that of the N-terminal region of the replication helicase, DnaB. This structural motif is likely to be involved in the inter-domainal regulation of ATPase activity for both Mu A and DnaB. The approach described here for structural determination in high salt may be generally applicable for proteins that do not crystallize and that are plagued by solubility problems at low ionic strength.

PubMed: 11060014
DOI: 10.1093/emboj/19.21.5625

実験手法

SOLUTION NMR