1F5U

SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA

1F5U の概要

• エントリーDOI: 10.2210/pdb1f5u/pdb
• 分子名称: RNA (5'-R(*GP*GP*UP*GP*GP*GP*AP*GP*AP*CP*GP*UP*CP*CP*CP*AP*CP*C)-3') (1 entity in total)
• 機能のキーワード: retrovirus, moloney murine leukemia virus, dimerization, tetraloop, stem-loop, kissing dimer, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 11605.04

構造登録者

Kim, C.-H.,Tinoco Jr., I. (登録日: 2000-06-16, 公開日: 2000-08-21, 最終更新日: 2024-05-22)

主引用文献

Kim, C.H.,Tinoco Jr., I.
A retroviral RNA kissing complex containing only two G.C base pairs.
Proc.Natl.Acad.Sci.USA, 97:9396-9401, 2000

PubMed Abstract: The dimerization of viral RNA through noncovalent interactions at their 5' ends is a key step in the life cycle of retroviruses. In Moloney murine leukemia virus, three stem-loops are important in this process. One is a self-complementary tetraloop (H1), but the other two stem-loops (H2, H3) contain highly conserved GACG tetraloops that are not self-complementary sequences. Using two-dimensional NMR, we determined the structure of the H3 stem-loop. Surprisingly, it forms a stable, homodimeric kissing complex through only two intermolecular G small middle dotC base pairs. Cross-strand interactions of the adenines adjacent to the intermolecular G small middle dotC base pairs, plus unusual strong electrostatic interactions around the base pairs, contribute to the unexpected stability. This structure shows how even stem-loops without self-complementary sequences can facilitate the intermolecular recognition between two identical RNAs, and thus initiate dimerization and encapsidation of retroviral RNAs.

PubMed: 10931958
DOI: 10.1073/pnas.170283697

実験手法

SOLUTION NMR