1F59

IMPORTIN-BETA-FXFG NUCLEOPORIN COMPLEX

Summary for 1F59

• Entry DOI: 10.2210/pdb1f59/pdb
• Descriptor: IMPORTIN BETA-1, FXFG NUCLEOPORIN REPEATS (2 entities in total)
• Functional Keywords: protein-protein complex, transport protein receptor
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm : Q14974
• Total number of polymer chains: 4
• Total formula weight: 104144.48

Authors

Bayliss, R.,Littlewood, T.,Stewart, M. (deposition date: 2000-06-13, release date: 2000-08-16, Last modification date: 2024-02-07)

Primary citation

Bayliss, R.,Littlewood, T.,Stewart, M.
Structural basis for the interaction between FxFG nucleoporin repeats and importin-beta in nuclear trafficking.
Cell(Cambridge,Mass.), 102:99-108, 2000

PubMed Abstract: We describe the crystal structure of a complex between importin-beta residues 1-442 (Ib442) and five FxFG nucleoporin repeats from Nsp1p. Nucleoporin FxFG cores bind on the convex face of Ib442 to a primary site between the A helices of HEAT repeats 5 and 6, and to a secondary site between HEAT repeats 6 and 7. Mutations at importin-beta Ile178 in the primary FxFG binding site reduce both binding and nuclear protein import, providing direct evidence for the functional significance of the importin-beta-FxFG interaction. The FxFG binding sites on importin-beta do not overlap with the RanGTP binding site. Instead, RanGTP may release importin-beta from FxFG nucleoporins by generating a conformational change that alters the structure of the FxFG binding site.

PubMed: 10929717
DOI: 10.1016/S0092-8674(00)00014-3

Experimental method

X-RAY DIFFRACTION (2.8 Å)