1F3D

CATALYTIC ANTIBODY 4B2 IN COMPLEX WITH ITS AMIDINIUM HAPTEN.

1F3D の概要

• エントリーDOI: 10.2210/pdb1f3d/pdb
• 分子名称: CATALYTIC ANTIBODY 4B2, 2-(4-AMINOBENZYLAMINO)-3,4,5,6-TETRAHYDROPYRIDINIUM, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: catalytic antibody, amidinium, haptenic charge, immune system
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 95502.78

構造登録者

Golinelli-Pimpaneau, B.,Goncalves, O.,Dintinger, T.,Blanchard, D.,Knossow, M.,Tellier, C. (登録日: 2000-06-02, 公開日: 2000-09-13, 最終更新日: 2024-10-30)

主引用文献

Golinelli-Pimpaneau, B.,Goncalves, O.,Dintinger, T.,Blanchard, D.,Knossow, M.,Tellier, C.
Structural evidence for a programmed general base in the active site of a catalytic antibody.
Proc.Natl.Acad.Sci.USA, 97:9892-9895, 2000

PubMed Abstract: The crystal structure of the complex of a catalytic antibody with its cationic hapten at 1.9-A resolution demonstrates that the hapten amidinium group is stabilized through an ionic pair interaction with the carboxylate of a combining-site residue. The location of this carboxylate allows it to act as a general base in an allylic rearrangement. When compared with structures of other antibody complexes in which the positive moiety of the hapten is stabilized mostly by cation-pi interactions, this structure shows that the amidinium moiety is a useful candidate to elicit a carboxylate in an antibody combining site at a predetermined location with respect to the hapten. More generally, this structure highlights the advantage of a bidentate hapten for the programmed positioning of a chemically reactive residue in an antibody through charge complementarity to the hapten.

PubMed: 10963661
DOI: 10.1073/pnas.97.18.9892

実験手法

X-RAY DIFFRACTION (1.87 Å)