1F2S

CRYSTAL STRUCTURE OF THE COMPLEX FORMED BETWEEN BOVINE BETA-TRYPSIN AND MCTI-A, A TRYPSIN INHIBITOR OF SQUASH FAMILY AT 1.8 A RESOLUTION

「1MCU」から置き換えられました

1F2S の概要

• エントリーDOI: 10.2210/pdb1f2s/pdb
• 関連するPDBエントリー: 1MCT
• 分子名称: TRYPSIN, TRYPSIN INHIBITOR A, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: proteinase-inhibitor complex, trypsin, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Bos taurus (cattle); 詳細
• 細胞内の位置: Secreted, extracellular space: P00760; Secreted: P10295
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26616.32

構造登録者

Zhu, Y.,Huang, Q.,Qian, M.,Jia, Y.,Tang, Y. (登録日: 2000-05-29, 公開日: 2000-06-05, 最終更新日: 2024-10-16)

主引用文献

Zhu, Y.,Huang, Q.,Qian, M.,Jia, Y.,Tang, Y.
Crystal structure of the complex formed between bovine beta-trypsin and MCTI-A, a trypsin inhibitor of squash family, at 1.8-A resolution.
J.Protein Chem., 18:505-509, 1999

PubMed Abstract: The stoichiometric complex formed between bovine beta-trypsin and Momordica charantia, Linn. Cucurbitaceae trypsin inhibitor A (MCTI-A) was crystallized and its X-ray crystal structure was refined to a final R value of 0.179 using data of 7.0- to 1.8-A resolution. Combination with results on the complex of MCTI-A with porcine trypsin gives the sequence of MCTI-A definitely, of which 13 residues are conserved compared with other squash family trypsin inhibitors. Its spatial structure and the conformation of its primary binding segment from Cys3I (P3) to Glu7I (P3'), which contains a reactive scissile bond Arg5I C-Ile6I N, were found to be very similar to the other squash family proteinase inhibitors.

PubMed: 10524768
DOI: 10.1023/A:1020690931043

実験手法

X-RAY DIFFRACTION (1.79 Å)